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Previously selected VHHs from naïve or immune libraries were subcloned into pAOCAT (Monegal et al., 2012) using the NcoI and NotI restriction sites. Chloramphenicol resistance assay was performed using BL21 (DE3) cells transformed with the pAOCAT-VHH fusion constructs. (See the Appendix for more details).
Copyright and License information: ©2016, Moutel et al
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