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The sorted TAM or in vitro‐treated macrophages were fixed with 4% paraformaldehyde, stained with oil red O (Sangon Biotech) for 1 hour at room temperature (RT) according to the manufacturer’s protocols, and then imaged with light microscopy. For BODIPY 493/503 staining, the slides were stained with BODIPY 493/503 (Thermo Fisher Scientific) at 0.5ug/mL for 15 min at RT, as previously described. 22 Slides were imaged with immunofluorescence microscopy.
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