Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Determination of limit of blank (LOB) and limit of detection (LOD)
Request a Protocol
Ask a
question
Favorite
In order to determine the false positive rate for each assay and accurately determine the limit of detection, we analyzed cell-free DNA extracted from plasma donated by 20 healthy volunteers (blank samples). The LOB was calculated as described by Armbruster and Pry31, where: LOB = meanblank + 1.645*SDblank.
After establishing the LOB, we calculated the lowest analyte concentration likely to be reliably distinguished from the background (LOD) as follow: LOD = LOB + 1.645*(SD low concentration sample)31.
Copyright and License information: The Author(s) ©2020
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
