Cell lines and culture conditions

A-549 (human lung carcinoma), CCC-222 (human colorectal carcinoma), DU-145 (human prostatic carcinoma), MCF-7 (human breast carcinoma), K-562 (human leukemic carcinoma), and PC-3 (human prostatic carcinoma) cell lines were procured originally from ATCC. All cell lines were maintained in RPMI 1640 culture medium (Biochrom, Germany), supplemented with 10% fetal bovine serum (FBS) (FBS; Biochrom, Germany), penicillin (100 U/mL) and streptomycin sulphate (100 mg/mL) (Biochrom, Germany). Cells were incubated at 37°C in 5% CO₂, 95% air in a humidified incubator.