MTT cytotoxicity assay

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay was used to determine cell viability. LNCaP and PC3 cells were seeded in 96-well plates and incubated at 37^oC, 5% CO₂ to allow exponential growth. Medium was then replaced by fresh medium containing drugs, oleic acid, or antibodies at the required concentrations in triplicate wells. MTT was added to a final concentration of 0.5 mg/mL and incubated for 2 hours. Cells were then solubilized by addition of DMSO and the absorbance read at 570 nm using a Tecan M2000 plate reader.