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Cell-cycle analysis by flow cytometry
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Cells were plated at a density such that they would be 50% confluent on the day of analysis. Cells were trypsinized and fixed in cold 70% ethanol for 10 min and then stained with propidium iodide solution (1 mM) at room temperature for 30 min. The stained cells were analyzed using BD Accuri C6 flow cytometer with excitation at 488 nm and emission at 617 nm.
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