Measurements of fluorescence anisotropy, fluorescence lifetime, as well as absorption, excitation and emission spectra.

We determined fluorescence anisotropy, fluorescence lifetime, as well as excitation and emission spectra using an ISS K2 multifrequency fluorometer in bulk measurements. All experiments were performed at room temperature (21–23° C). Instrument settings for the excitation and emission spectra are listed in Supplementary Table 5, settings for the fluorescence anisotropy measurements are listed in Supplementary Table 6, and settings for the fluorescence lifetime measurements are listed in Supplementary Table 7. For the absorption spectra we used an Eppendorf Spectrophotometer (UV-Vis BioSpectrometer) and measured the absorbance from 240 nm to 800 nm. For all samples we used a concentration of 500 nM. In addition, we normalized the absorption spectra based on the 260 nm absorbance.