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Cell apoptosis detected by Caspase-3/7 assay kit
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Caspase-3/7 activities were measured using the Caspase-Glo® Glo Assay kit (Promega) according to the manufacturer's protocol. Cells were plated in triplicate in 96-well cell culture plates. After 3 days siRNA transfection, cells were incubated with 100 µl caspase-Glo reagent at room temperature for 30 minutes. Assays were measured by detection with a fluorescence microplate reader (TECAN infinite M2009MR).
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