Protein localization and fluorescence quantification

Images for protein localization experiments before or after injury were acquired on a Zeiss LSM 800 confocal microscope. Maximum intensity projections of z-stacks were used for all localization analyses. UAS-dsh-GFP was quantified on a binary basis with branch points that had a single dsh-GFP puncta or none. For the nonpunctate markers UAS-γTub-GFP or UAS-Axin-GFP, the area between branch points (nBP) and branch point (BP) values were collected and measured as described in Weiner and colleagues [54]. y-axis values shown on the graphs indicate the BP − nBP fluorescent value.