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In vitro cytotoxicity was determined using the CellTiter 96 nonradioactive cell proliferation colorimetric assay (MTT assay; Promega) in primary human peripheral blood mononuclear cells (PBMC) and in human T lymphoblast (CEM) and human hepatocellular carcinoma (HepG2) cell lines. Toxicity levels were measured as the concentration of test compound that inhibited cell proliferation by 50% (50% cytotoxic concentration, CC50).
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