MTT assay

An MTT assay was performed to investigate the effect of miR-214 and CADM1 on melanoma cell viability. A375 and A2058 cells (5×10³ cells per well) were seeded on 96-well plates and incubated at 37°C for 24, 48 and 72 h. Then, 20 µl MTT solution (Sigma-Aldrich; Merck KGaA) was added to each well and incubated at room temperature for another 4 h. Then, the culture medium with MTT was discarded, 150 µl DMSO was added and cells were agitated for 10 min at room temperature. The absorbance was measured at 490 nm with a standard microplate reader, and cell viability was analyzed.