Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

In vivo xenograft model

XD Xinyue Du
SW Shuangmiao Wang
XL Xingyan Liu
TH Tao He
XL Xiangui Lin
SW Simin Wu
DW Dan Wang
JL Jiao Li
WH Wenhua Huang
HY Huiling Yang
request Request a Protocol
ask Ask a question
Favorite

Pathogen-free BALB/c-nu thymus-free nude mice (4–5 weeks old, male) were used, with two groups of five mice each (groups lv-miR-1307-5p and lv-miR-1307-5p inhibitor, each compared with lv-NC in the same animal). The overexpression stable strain and negative control stable strain cells were separately inoculated subcutaneously to the left and right sides of one group of mice (5 × 106 = number of cells injected per nude mouse), and the inhibition stable strain and negative control stable strain cells were separately inoculated subcutaneously to the left and right sides of the other group of mice (5 × 106 = number of cells injected per nude mouse). Eight days later, tumors were established. Tumor size and body mass were measured every five days. The volume was calculated as (length × width × width)/2, and the tumor tissue was collected for analysis.

Copyright and License information: The Author(s) ©2020
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A