2.10. EPR Spin Trapping Experiments

The generation of paramagnetic intermediates was monitored by cw-EPR spectroscopy using the EMX spectrometer (Bruker). The EPR spectra were measured with following experimental parameters: X-band, room temperature, microwave frequency, 9.431 GHz; modulation frequency, 100 kHz; field modulation amplitude, 2 G; time constant, 10 ms; scan time, 41s (5 scans). Distilled and deionised water was used for the preparation of DMSO/water solutions. The spin trapping agent 5,5-dimethyl-1-pyrroline N-oxide (DMPO; Sigma-Aldrich, Schnelldorf, Germany) was distilled prior to the application.