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ELISA-based malaria-specific antibody assay

WH William O. Hahn
MP Marion Pepper
WL W. Conrad Liles
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96 well ELISPOT plates (Millipore) were coated overnight at 4 C with MSP1+ protein at 1 µg/mL. Plates were then blocked with 5% dehydrated milk prior to sample incubation. Plates were incubated with serially diluted serum. Bound antibodies were detected using either IgM Biotin (Clone II/41) or IgG2 c Biotin (Clone 5.7) followed by Streptavidin-HRP (BD). Absorbance was measured at 450 nm using an iMark Microplate Reader (Bio-Rad).

Copyright and License information:  ©2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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