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A human prostaglandin E2 (PGE2)-specific ELISA kit was used in accordance with the manufacturer’s protocol (catalog no. 514010; Cayman Chemical). After the applied treatments, the culture media were collected, and PGE2 levels in the culture media were measured by ELISA. PGE2 levels were normalized to the protein concentrations in the cell lysates. Normalized PGE2 values from the treatment groups are represented as relative values based on comparison to the control group.
Copyright and License information: ©2020 Zhang, Zhu, Chang and Leung.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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