The in vitro drug release profile of doxorubicin from vesicles was quantified using the dialysis method as reported in our previous study [20]. A dialysis bag with a molecular weight cutoff of 10 kDa (Solarbio Science & Technology Co., Shanghai, China) containing 1 mL samples were incubated in 200 mL release buffer (PBS; pH 7.4) at 25 °C for 24 h (the buffer was gently stirred on a magnetic stirrer). Approximately 0.5 mL of release buffer was taken (0.5 mL of fresh buffer was supplemented concurrently) at each time point (0.25, 0.5, 1, 2, 3, 4, 6, 8, 12, and 24 h, respectively) and immediately measured via HPLC to determine the concentration of released doxorubicin.
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