Cell Death Determined by Lactate Dehydrogenase Assay

Xia Zhu; Haiyan Liu; Yuan Liu; Yajing Chen; Yaowu Liu; Xiaoxing Yin

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Cell Death Determined by Lactate Dehydrogenase Assay

XZ Xia Zhu

HL Haiyan Liu

YL Yuan Liu

YC Yajing Chen

YL Yaowu Liu

XY Xiaoxing Yin

This method is extracted from research article: Front Pharmacol, Aug 2020

The Antidepressant-Like Effects of Hesperidin in Streptozotocin‐Induced Diabetic Rats by Activating Nrf2/ARE/Glyoxalase 1 Pathway

DOI: 10.3389/fphar.2020.01325

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Previous findings have shown that the activity of the LDH released from damaged cells is proportional to the number of damaged neurons (McCord and Fridovich, 1969; Wang et al., 2015). After exposure to the drugs, 50 μl of the medium was removed, and the amount of LDH released from the cells was determined using a LDH kit (Solarbio Life Sciences, China) according to the manufacturer’s instructions. The absorbance of the samples was read spectrophotometrically at 450 nm. The results are expressed as the percentage of LDH released relative to that released from the control cells.

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