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2.8. Aldehyde dehydrogenase (ALDH) Assay

WL Wan-Chun Li
HC Hsin-Yung Chen
CC Chun-Pin Chiang
HC Hsin-Ming Chen
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ALDH activity was detected by ALDEFLUOR™ Kit (Stemcell technologies Inc., Vancouver, BC, Canada). The detected cells were added into ALDEFLUOR™ Assay Buffer as the test group, and the buffer contained BODIPY-aminoacetaldehyde (BAAA). ALDH+ cells can catalyze BAAA to be a fluorescent product, BODIPY-aminoacetate. Diethylaminobenzaldehyde (DEAB) is a specific inhibitor that is used as a negative control to define background ALDH activity. The assay was done by BD FACSCalibur™ flow cytometry (BD Bioscience, Franklin Lakes, New Jersey, USA).

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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