It has previously been reported that the level of β-hexosaminidase and histamine from RBL-2H3 cells activated by IgE–FcεRI complex were detected [45]. The cellular cytotoxicity of viridicatol was measured using MTT (3-(4,5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide) assay.
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