4.5. Determination of Histamine and β-Hexosaminidase Release in RBL-2H3 Cells

ZS Zhendan Shu
QL Qingmei Liu
CX Cuiping Xing
YZ Yafen Zhang
YZ Yu Zhou
JZ Jun Zhang
HL Hong Liu
MC Minjie Cao
XY Xianwen Yang
GL Guangming Liu
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It has previously been reported that the level of β-hexosaminidase and histamine from RBL-2H3 cells activated by IgE–FcεRI complex were detected [45]. The cellular cytotoxicity of viridicatol was measured using MTT (3-(4,5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide) assay.

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