Western blots

Primary antibodies were diluted in Licor blocking solution/PBST with a final Tween-20 concentration of 0.1%, except for anti-phospho Aurora and anti-Histone H3S10ph, which had no Tween-20. The following antibodies and antibody dilutions were used: anti-INCENP (raised against C-terminal peptide CSNRHHLAVGYGLKY) (5.5 μg/ml), anti-Aurora B (Kelly et al., 2007 ; 5 μg/ml), anti-Borealin-2 (Dasra A; Kelly et al., 2007 ; 5 μg/ml), anti-Survivin (Tseng et al., 2010 ; 12 μg/ml), anti-phospho Aurora (Phospho-Aurora A [Thr288]/Aurora B [Thr 232]/Aurora C [Thr198] 2914, Cell Signaling Technology; 1:200), anti-Histone H3S10ph (6G3, 9706, Cell Signaling Technology; 1:500), anti-Histone H3T3ph (2162-1, Epitomics; 1:10,000), anti-GFP (11814460001, Sigma Aldrich; 1:1000), anti-PP2A-C (05-421, Millipore; 1:1000), Sgo1 (Boyarchuk et al., 2007 ; 1:250), and anti-alpha-tubulin (DM1, Sigma; 1:20,000). Secondary antibodies from Licor were used (Licor goat anti-Rabbit 800 nm and Licor goat anti-mouse 680 nm), as was the Licor imaging system to scan membranes.