Inhibition of glutathione synthesis and addition of GSH-MEE

PS Paul Souter
JV John Vaughan
KB Kerry Butcher
AD Adam Dowle
JC Jim Cunningham
JD James Dodd
MH Michael Hall
DW Darren Wilson
AH Alan Horner
PG Paul Genever
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CFU-f from primary MSCs were cultured with Ag+ (0, 1, 5 and 10 μM) in the presence/absence of a pharmacological inhibitor of γ-glutamylcysteine synthetase, l-buthionine-sulfoxamine (BSO, 0.01, 0.1 1 μM). Recovery the glutathione pathway in BSO treated cultures was through media supplementation with 2 mM GSH-MEE. Media were changed twice per week with CFU-f number assessed at 14 days.

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