4.8. Adoptive Transfer of Tregs

CD4⁺CD25⁺ regulatory T cells were isolated, as mentioned above. TLR2 KO mice were injected i.v. with 1.0 × 10⁵ Tregs from WT mice or TLR2 KO mice one day before imiquimod application. The number of transferred Tregs was determined in reference to similar experiments using Bregs [50]. Messenger RNA expression in the lesional skin two days after imiquimod application was further analyzed as mentioned above.