4.3. SiRNA Knockdown of AHR

HUVEC were transfected with siRNA control (Negative Universal Control, Stealth™ RNAi, Life Technologies, Courtaboeuf, France), or a pool containing 3 Silencer^® Select siRNA directed against AHR (1200, 1999 and 1998, Life Technologies, Courtaboeuf, France) by magnetofection using SilenceMag beads (OZ Biosciences, France), according to the manufacturer’s instructions. Cells were detached with trypsin-EDTA solution 24 h post-transfection and plated at 1.5 × 10⁶ cells/mL in EGM2 medium. Forty-eight hours post-transfection, cells were subjected to static, shear stress and/or IS conditions.