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Mouse MIN6 insulinoma cells were cultured in DMEM, as previously reported (15). DMEM was supplemented with 15% fetal bovine serum, antibiotics (100 units/mL penicillin and 100 μg/mL streptomycin), 1 mM sodium pyruvate, 10 mM HEPES, and 50 μM β-mercaptoethanol. The cells were maintained at 37°C in an atmosphere of 5% CO2 and 100% humidity.
Copyright and License information: ©2019 Endocrine Society
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