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Cell cycle arrest and release

HL Han Lei
KW Kun Wang
TJ Tongying Jiang
JL Jingjing Lu
XD Xue Dong
FW Feilong Wang
QL Qiang Li
LZ Liming Zhao
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SK-MES-1 cells in the logarithmic growth phase were seeded in 6-well plates at 5 × 105 cells/well with RPMI-1640 medium containing 10% FBS and cultured overnight under normal conditions. Then, nocodazole (Sigma, CA, USA) at a final concentration of 1 μg/ml was added to the medium, and the cells were cultured for 18 h. Next, cycloheximide (CHX, Sigma, CA, USA) at a final concentration of 10 μg/mL was added to the medium, and the cells were cultured for another 5 h; some cells were collected every hour. UbcH10 and KIAA0101 expression in the cells harvested at different time points was detected by western blotting.

Copyright and License information: The Author(s) ©2020
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