Effect of changes in 11β-HSD1 expression on the release of collagen in HSCs

Cells from each group were collected and the concentration of collagen was measured using the ProCell collagen colorimetric assay kit, according to the manufacturer's protocol. Briefly, Sircol dye reagent was added to the cell culture supernatants (1,000 × g; 10 min; room temperature), stirred for 20 min at room temperature and centrifuged at 15,000 × g for 10 min at room temperature. The absorbance of the bound dye was measured at a wavelength of 555 nm on a spectrophotometer.