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Agonist-induced β-arrestin recruitment was determined using “Tango” assay [53, 54]. HTLA cells expressing β-arrestin-TEV protease fusion and tetracycline transactivator-driven luciferase reporter [53] were transfected via CaPO4 [52] with opioid receptor-Tango vectors (FLAG-KOR-Tango, FLAG-MOR-Tango, or FLAG-DOR-Tango; contributed to Addgene by Dr. Bryan Roth) with or without WT hRGS12, Rgs12E740K, or Rgs12R1206F vectors. Additional procedures are detailed in Supplementary Methods.
Copyright and License information: American College of Neuropsychopharmacology ©2019
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