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Aβ peptides were selected in Saccharomyces cerevisiae, produced in Escherichia coli and purified as previously described (D’Angelo et al., 2013). Following purification and dialysis, oG37C were isolated through size-exclusion chromatography (Vignaud et al., 2013). After quantification by Bradford protein assay, peptides were frozen in liquid nitrogen and stored at −80°C until further use.
Copyright and License information: ©2020 Feuillie, Lambert, Ewald, Azouz, Henry, Marsaudon, Cullin, Lecomte and Molinari.
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