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The effect of bergenin on the viability of HK-2 and Caco-2 cells was evaluated using the CCK8 assay. Cells were treated with different concentrations of bergenin, followed by incubation with 10% CCK-8 (Dojindo, Kumamoto, Japan) in 100 μL of high-glucose serum-free DMEM for 4 h at 37°C. Absorbance at 450 nm was measured on a microplate reader (ELX808; BioTek, Winooski, VT, United States).
Copyright and License information: ©2020 Chen, Ye, Zhu, Zhang, Jiang, Lu and Wu.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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