Quantification of reactive oxygen species (ROS).

Blood vessels were perfused, retinas were isolated and incubated in Krebs-HEPES buffer (with 5mmol/L glucose) for 25 min at 37°C in 5% CO₂. Luminescence was measured 5 min after the addition of 0.5 mmol/L lucigenin, as previously described⁶. Alternatively, 2’,7’-dichlorodihydrofluorescein diacetate (CFDA), was used as an indicator for ROS by flow cytometry analysis as previously described¹². Anti-IL-17RC was added 2h prior to IL-17A stimulation.