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Dual Luciferase Reporter Assay

CG Chun-Yi Guan
ST Shi Tian
JC Jing-Li Cao
XW Xue-Qin Wang
XM Xu Ma
HX Hong-Fei Xia
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Detection for the reporter assays was performed using the Promega dual luciferase assay (Promega, Madison, USA). Briefly, 10 μL of cell lysate was pipetted into the reaction tube and 30 μL of LAR II solution was added to the reaction tube, mixed, and the firefly luciferase activity was measured. Afterwards, 30 μL of Stop & Glo solution was added to the reaction tube and mixed to detect Renilla luciferase activity. Each treatment was performed in triplicate and in three independent experiments. The results have been expressed as relative luciferase activity (firefly luciferase/Renilla luciferase).

Copyright and License information:  ©2020 Guan et al.
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