Measured and calculated parameters

Hemodynamic variables (MABP, HR) were monitored and recorded to calculate rate pressure product (PRP= MABP x HR / 1000) as an indirect index of myocardial oxygen consumption.^12,13 PRP was calculated after the surgical procedure (baseline), before and at the end of ischemia, at the beginning and end of the reperfusion.

The arrhythmia parameters were also evaluated from the ECG recordings of the rats in accordance with the Lambeth conventions at the end of the experimental protocols.¹⁸ The incidence of ventricular tachycardia (VT) and ventricular fibrillation (VF) were determined in each group.