2.1. Yeast Strains and Growth Conditions

SL Shawn M. Lyons
PI Pavel Ivanov
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The strains used in this study are described in Supplementary Table S1. The strains knocked out for specific genes were obtained from the Yeast Knockout Collection. Strains were grown on YPD (yeast extract peptone dextrose) or synthetic media (VWR Glucose 2%, Difco Yeast Nitrogen Base 0.17%, Fisher Ammonium sulfate 5 g/L, appropriate amino acids and nucleotides). All strains were grown at 30 °C in shaking water baths. A standard lithium acetate technique was used for yeast transformations.

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