2.6. Single enrichment and gene set enrichment analysis

Blast2GO results, including GO term annotation and KEGG pathway information, were used for functional analysis of differentially expressed genes. GO term enrichment was highlighted for every analysis (drought stress response as well as constitutive accumulation) by comparison between functionalities identified in genes significantly upregulated and downregulated. GO term enrichment was analyzed by using FatiGO (Al‐Shahrour et al., 2007) implemented in the OmicsBox software. Significant enrichment of GO terms was considered for p‐values <.05. Venn diagrams were drawn using Venny 2.1 (Oliveros, 2015). KEGG pathway enrichment analysis was carried out using GSEA software (Subramanian et al., 2005). All genes preranked according to Kal's statistics were included in order to analyze every comparison as a global system. Analyses were run with 1,000 permutations of gene sets. Pathways with p‐values <.05 were considered positively or negatively correlated.