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The cells were seeded in 24-well plates at the density of 5000 cells/well/600μl media, and treated with DMSO or 10mM Enz for 0, 1, 2 and 4 days. To determine cell viability at each time point, 300μl of the MTT reagent (Sigma, Germany) was added to each well, and the cells were incubated for 2h. The medium was then removed, and 300μl DMSO was added to dissolve the formazan crystals. The optical density at 490 nm was measured by a microplate reader (BioTek, USA).
Copyright and License information: ©2020 Wang et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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