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Total RNA was isolated using the RNeasy Extraction Kit (Quiagen). To quantify Myc expression levels, equal amounts of cDNA were synthesized using the SuperScript™ III First-Strand Synthesis System (Invitrogen) and mixed with the Power SYBR Green PCR master mix (Applied Biosystems, Carlsbad, CA) and 5 pmol of both forward and reverse primers. GAPDH was amplified as an internal control. The sequences of the human primers used for qPCR, listed from 5’ to 3’, were:
MYC, (forward) CCTACCCTCTCAACGACAGC, (reverse) CTCTGACCTTTTGCCAGGAG; ACTB (β-actin), (forward) AATCTGGCACCACACCTTCTAC, (reverse) ATAGCACAGCCTGGATAGCAAC.
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