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The 14C-EF5 binding assay was performed as described previously 51. In brief, 1x105 cells/well of a 96-well plate were incubated with 2 mM 14C-EF5 under oxic or anoxic conditions for 4 h. Reduced and bound 14C-EF5 adducts were measured in the trichloroacetic acid fixed cell pellets using a scintillation counter.
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