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Generating Ag-experienced CD8 T cells
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Mice received 104 naïve Thy disparate P14 or OT-I CD8 T cells prior to infection. Attenuated wild-type DPL1942 and recombinant L. monocytogenes expressing ovalbumin and glycoprotein 33 (LM or LM-OVA/GP33, respectively) strains were injected i.v. at 1×107 CFU/mouse. Virulent LM strain 10403s was injected i.v at 1×106 CFU/mouse. LCMV Armstrong was injected i.p. at 2×105 PFU/mouse.
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