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Evaluation of IL-1β, IL-6 and TNF-α expression in brain tissue by ELISA
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Brain tissue was thoroughly homogenized in ice cold PBS, before centrifugation at 500 × g at 4°C for 15 min. The supernatant was then assayed for IL-1β (orb79117; Biorbyt Ltd.), IL-6 (orb79123; Biorbyt Ltd.) and TNF-α (orb79138-480; Biorbyt Ltd.) according to manufacturer's protocols. The results were obtained by measuring absorption at 450 nm on a microplate reader (Model 680, Bio-Rad Laboratories, Inc.).
Copyright and License information: ©2019 Xiang et al.
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