ChAdOx1 Zika (also known as ChAdOx1 Zika prME ΔTM) was produced as previously described (35) and used as the off-target control vaccine in our challenge experiments. The MAYV-IRES vaccine were previously developed (36), by inserting an IRES in the genome of MAYV and passaging, respectively. Unrelated ChAdOx1 dengue NS1 (unpublished) was used as the mock vaccine in the immunogenicity studies.
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