Mice

C57BL/6 were obtained from Charles River (Germany). Pik3cg^–/– (Pik3cg^tm1Pen ) mice (Sasaki et al., 2000) were provided by J. Penninger. Pik3cg^–/– (Pik3cg^tm1Wym) [12] and PI3Kγ-kinase-dead (KD) (Pik3cg^tm1Ehi) mice [13] were backcrossed for 20 and 15 generations to C57BL/6, respectively, and provided by E. Hirsch. Csf2rb^−/− mice were provided by B. Becher, Zürich. Csf2ra^A302Gfs*1 (Csf2ra^m1Kopf) mice were initially derived from Pik3cg^tm1Pen, backcrossed for an additional 3 generations to C57BL/6 and carry wild-type alleles of Pik3cg. All animals were housed in individually ventilated cages under specific pathogen–free conditions and were used for experiments at between 7 and 12 weeks of age. All animal experiments were approved by the local animal ethics committees and were performed according to local guidelines and Swiss animal protection law.