RNA transcriptome sequencing analysis

The RNA transcriptome sequencing analysis was performed by RiboBio Biotechnology Corporation (Shanghai, China) using HiSeq technology. To assess molecular signalling pathway changes after IR treatment in C. elegans, the data were divided into three comparisons, containing 0 Gy versus 200 Gy (comparison A), 0 Gy versus 400 Gy (comparison B), 200 Gy versus 400 Gy (comparison C), the RNA-seq and alignment results shown in ESI Table 1.^† Data analysed by canonical KEGG pathways, GO, and differential gene expression; these analyses were performed by Cloud Exploring Biotechnology (Guangzhou, China).