4.2. Cell Lines

Human lung cancer NCI-H1573, A549, NCI-H1299, and NCI-H1975 cells were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). A549 cells were routinely cultured in F12 Kaighn’s medium (Invitrogen, Carlsbad, CA, USA), and NCI-H1573, NCI-H1299, and NCI-H1975 were cultured in RPMI 1640 medium (Invitrogen, Carlsbad, CA, USA). The media used for the routine cell culture were supplemented with 10% (v/v) fetal bovine serum (FBS) and a 1% (w/v) combination of penicillin, streptomycin, and neomycin (Invitrogen, Carlsbad, CA, USA). The cultures were incubated in 75 cm² vented culture flasks at 37 °C in 5% CO₂/95% humidified air. The cells were trypsinized after they were 80–90% confluence and seeded onto the appropriate well plates.