2.9. Flow cytometry analysis and magnetic cell sorting

Cells with different treatments were resuspended at 1 × 10⁶ cells per mL in PBS containing 2% FBS and then subsequently stained with PE‐conjugated CD133 antibody (BioLegend) for 30 minutes on ice in dark. After washing with ice‐cold PBS, each sample was analysed by flow cytometry (BD Bioscience). The positive CD133 subpopulations of Capan‐2 cells were sorted from using MACS separation (Miltenyi Biotec) according to the manufacturer’s protocol as described previously. 25