Individual shRNA vectors used were obtained from the Mission TRC library (Sigma): shCCND1 #1 (TRCN0000295876), shCCND1 #2 (TRCN0000288598), shCDK4 #1 (TRCN0000000362), shCDK4 #2 (TRCN0000196698), shCCNE1 #1 (TRCN0000045301), and shCCNE1 #2 (TRCN0000045302). Overexpression vectors were obtained from the TRC3 ORF collections from TransOMIC and Sigma: pLX304-GFP, pLX304-CCND1, pLX317-GFP, and pLX317-CDK4. These above plasmids are provided by the Genetic Perturbation Service of Goodman Cancer Research Centre and Biochemistry at McGill University (Montreal, Quebec, Canada).
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