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Strains and culture conditions

XZ Xuechao Zhang
XG Xiaojun Guo
CW Cuihong Wu
CL Chengcui Li
DZ Dongdong Zhang
BZ Baocheng Zhu
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Bacillus subtilis Z-14, which significantly reduced the growth of Rhizoctonia cerealis (a wheat sheath blight pathogen), was originally isolated from soil sampled from a wheat rhizosphere [20]. Pichia pastoris GS115 and pPIC9K were used as the host and vector, respectively, for heterologous expression of the recombinant antifungal protein F2. Z-14 was grown on nutritive agar (NA) overnight at 37 °C. An aliquot of the overnight culture was inoculated into 50 ml of fermentation medium (20 g of sucrose; 10 g of tryptone; 2 g of KH2PO4; 0.05 g of CaCl2; 0.05 g of MgSO4·7 H2O; and 1000 ml of distilled water; pH 7.5) in an Erlenmeyer flask and cultured for 48 h at 37 °C with shaking at 220 rpm.

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