2.5. d‐ROMs FAST test

The d‐ROMs FAST test is a colorimetric determination. The kit consists of R1 reagent, a condensed solution of N,N‐diethyl‐para‐phenylendiamine (chromogen), R2 reagent, acetic buffer pH 4,8, and R3 reagent, a ferric ion solution. Ten microliters of blood plasma is added to 1 mL of working solution and the mixture is poured onto R1 reagent. The working solution consists of 10 μL of R3 reagents in 1 mL of pre‐dosed R2 reagent. The photometric reading was taken at 505 nm after 150 seconds of incubation at 37°C.

This test is based on the Fenton reaction and the rate of coloration is proportional to the peroxide concentration. The results are expressed in U. Carr., where 1 U. Carr. = 0.08 mg/dL of H₂O₂. The normal range is between 250‐300 U. Carr. ²⁰