Treatment of mDCs With HSV-1-Derived Particles

Mature DCs were incubated with HSV-1-derived particles as follows: 1 × 10⁶ cells were mock- or HSV-1-infected (MOI of 2), incubated with purified H-particles (MOI of 2) or L-particles (viral material corresponding to high MOI). For inactivation of marginal H-particle contaminations, L-particles were inactivated by three times applying 0.12 J/cm² in a Vilber Luormat. The infection was performed as described above. At 1 hpi, mDCs were transferred without centrifugation into DC medium containing 40 U/mL GM-CSF and 250 U/mL IL-4. Cells were harvested 24 hpi and prepared for flow cytometric analyses.