Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Light microscopy observation of localization of CgCrzA.
Request a Protocol
Ask a
question
Favorite
Fresh mycelia of the complemented transformants were cultured in liquid CM medium at 25oC for 24 h and stained with 10 μg/ml DAPI (Sigma-Aldrich, St. Louis, MO, USA). To evaluate the effect of CaCl2 and FK506 on localization of CgCrzA, 0.2 M CaCl2 or 10 μg/ml FK506 was, respectively, added to liquid CM medium for 30 min at 28oC before observation. Photographs were taken under a confocal laser scanning microscope (Zeiss, Oberkochen, Germany).
Copyright and License information: ©2020 The Korean Society of Plant Pathology
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
