SRPX2-FLAG-pCAG was generated by PCR-amplifying SRPX2 (NM_026838) from murine brain cDNA, and subcloning the PCR fragment with a C-terminal FLAG tag into a pCAG vector between the NotI and EcoRV sites. HA-C1qa, HA-C1qb, and HA-C1qc plasmids were generated by PCR-amplifying murine C1qa (NM_007572), C1qb (NM_009777), and C1qc (NM_007574) from murine brain cDNA, and cloning the fragments into a pCAG vector between the EcoRI and XhoI sites, inserting an N-terminal HA (YPYDVPDYA) epitope tag after the signal peptide.
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